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Rabbit polyclonal to TPST1
Predicted to work with:
Mouse, Cow, Dog
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TPST1.
This antibody gave a positive signal in HeLa and HepG2 whole cell lysates as well as in the following tissue lysates: Human lung; Mouse cerebellum; Rat cerebellum.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Belongs to the protein sulfotransferase family.
Golgi apparatus membrane.
Information by UniProt
Protein tyrosine sulfotransferase 1 antibody
Protein-tyrosine sulfotransferase 1 antibody
Western blot - TPST1 antibody (ab100972)
All lanes :
Anti-TPST1 antibody (ab100972) at 1 µg/ml
Lane 1 :
Lung (Human) Tissue Lysate
Lane 2 :
Cerebellum Mouse Tissue Lysate
Lane 3 :
Cerebellum Rat Tissue Lysate
Lane 4 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
50 kDa (
why is the actual band size different from the predicted?
Additional bands at:
27 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
TPST1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"