Overview

  • Product nameAnti-TPST1 antibody
  • Description
    Rabbit polyclonal to TPST1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TPST1.

  • Positive control
    • This antibody gave a positive signal in HeLa and HepG2 whole cell lysates as well as in the following tissue lysates: Human lung; Mouse cerebellum; Rat cerebellum.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).

Target

Anti-TPST1 antibody images

  • All lanes : Anti-TPST1 antibody (ab100972) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Cerebellum Rat Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-TPST1 antibody (ab100972)

This product has been referenced in:
  • Hewit KD  et al. The N-terminal region of the atypical chemokine receptor ACKR2 is a key determinant of ligand binding. J Biol Chem 289:12330-42 (2014). WB ; Human . Read more (PubMed: 24644289) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"