Anti-TPTE antibody (ab110920)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TPTE
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Two synthetic peptides, one derived from the last 50 amino acids of the protein (500 to 551) and the other in the middle of the protein between amino acids 200 to 250 of Human (UniProt ID: P56180).

Properties

Applications

Our Abpromise guarantee covers the use of ab110920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 64 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Could be involved in signal transduction.
  • Tissue specificity
    Exclusively expressed in testis.
  • Sequence similarities
    Contains 1 C2 tensin-type domain.
    Contains 1 phosphatase tensin-type domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 44 antibody
    • CT44 antibody
    • EC=3.1.3.48 antibody
    • HGNC:12023 antibody
    • PTEN2 antibody
    • Putative tyrosine protein phosphatase antibody
    • Putative tyrosine-protein phosphatase TPTE antibody
    • TPTE antibody
    • TPTE_HUMAN antibody
    • Transmembrane phosphatase with tensin homology antibody
    • Tumor antigen BJ HCC 5 antibody
    • Tumor antigen BJ-HCC-5 antibody
    see all

References

ab110920 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for submitting your Abreview. I trust that you have been informed that it has been "published" on the datasheet for ab110920. I agree that the vial of ab110920 is reacting specifically with a protein that is not TPTE. I am very sorry f...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary Cortical Neurons)
Specification
Primary Cortical Neurons
Fixative
Formaldehyde
Permeabilization
Yes - saponin (0.4%)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Mario Rosasco

Verified customer

Submitted Oct 05 2011

Application
Western blot
Sample
Mouse Recombinant protein (HEK293 cells expressing mouse Tpte protein)
Loading amount
20 µg
Specification
HEK293 cells expressing mouse Tpte protein
Treatment
CaPO4 transfected with mouse Tpte for 72 hours
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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