Overview

  • Product nameAnti-TPTE antibody
    See all TPTE primary antibodies
  • Description
    Rabbit polyclonal to TPTE
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: DGLPLYDDVK VQFFYSNLPT YYDNCSFYFW LHTSFIENNR LYLPKNELDN, corresponding to C terminal amino acids 468-517 of Human TPTE

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab32976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCould be involved in signal transduction.
  • Tissue specificityExclusively expressed in testis.
  • Sequence similaritiesContains 1 C2 tensin-type domain.
    Contains 1 phosphatase tensin-type domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 44 antibody
    • CT44 antibody
    • EC=3.1.3.48 antibody
    • HGNC:12023 antibody
    • PTEN2 antibody
    • Putative tyrosine protein phosphatase antibody
    • Putative tyrosine-protein phosphatase TPTE antibody
    • TPTE antibody
    • TPTE_HUMAN antibody
    • Transmembrane phosphatase with tensin homology antibody
    • Tumor antigen BJ HCC 5 antibody
    • Tumor antigen BJ-HCC-5 antibody
    see all

Anti-TPTE antibody images

  • Anti-TPTE antibody (ab32976) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling TPTE with ab32976 at 4-8µg/ml. Positively labelled cells (indicated by arrows) are epithelial cells of pancreatic acinus. Magnification: 400X.

References for Anti-TPTE antibody (ab32976)

ab32976 has not yet been referenced specifically in any publications.

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