Overview

  • Product name
  • Description
    Goat polyclonal to TPTE
  • Specificity
    This antibody is expected to recognise all reported isoforms (NP_954870.2, NP_954868.1 and NP_954869.1). It may cross-react with the almost identical TPTE2 (GeneID 93492; NP_570141.2, NP_954864.1, NP_954863.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    ERRTDKTHSEKFQ

    (Human) from the internal region of the protein sequence according to NP_954870.2; NP_954868.1; NP_954869.1.

  • Positive control
    • Human testis lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab77194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/16000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Could be involved in signal transduction.
    • Tissue specificity
      Exclusively expressed in testis.
    • Sequence similarities
      Contains 1 C2 tensin-type domain.
      Contains 1 phosphatase tensin-type domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 44 antibody
      • CT44 antibody
      • EC=3.1.3.48 antibody
      • HGNC:12023 antibody
      • PTEN2 antibody
      • Putative tyrosine protein phosphatase antibody
      • Putative tyrosine-protein phosphatase TPTE antibody
      • TPTE antibody
      • TPTE_HUMAN antibody
      • Transmembrane phosphatase with tensin homology antibody
      • Tumor antigen BJ HCC 5 antibody
      • Tumor antigen BJ-HCC-5 antibody
      see all

    Anti-TPTE antibody images

    References for Anti-TPTE antibody (ab77194)

    ab77194 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Recombinant protein (HEK293 cells expressing mouse Tpte protein)
    Loading amount
    20 µg
    Specification
    HEK293 cells expressing mouse Tpte protein
    Treatment
    CaPO4 transfected with mouse Tpte for 72 hours
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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