The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 86 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionSpindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Tissue specificityExpressed in lung carcinoma cell lines but not in normal lung tissues.
Sequence similaritiesBelongs to the TPX2 family.
Developmental stageExclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
ab115628, at 10 µg/ml, staining TPX2 in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Following primary incubation, slides were incubated with biotinylated goat anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-TPX2 antibody [18D5] (ab115628)
has not yet been referenced specifically in any publications.
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