Overview

  • Product name
    Anti-TPX2 antibody [18D5]
    See all TPX2 primary antibodies
  • Description
    Mouse monoclonal [18D5] to TPX2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-220 of Human TPX2.

  • Positive control
    • Human testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 86 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
  • Tissue specificity
    Expressed in lung carcinoma cell lines but not in normal lung tissues.
  • Sequence similarities
    Belongs to the TPX2 family.
  • Developmental stage
    Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20ORF1 antibody
    • C20orf2 antibody
    • Chromosome 20 Open Reading Frame 1 antibody
    • Differentially expressed in cancerous and non-cancerous lung cells 2 antibody
    • Differentially expressed in cancerous and noncancerous lung cells 2 antibody
    • Differentially expressed in lung cells 2 antibody
    • Differentially expressed in lung cells antibody
    • DIL 2 antibody
    • DIL-2 antibody
    • DIL2 antibody
    • FLS353 antibody
    • GD:C20orf1 antibody
    • HCA 519 antibody
    • HCA519 antibody
    • HCTP 4 antibody
    • HCTP4 antibody
    • Hepatocellular carcinoma associated antigen 519 antibody
    • Hepatocellular carcinoma-associated antigen 519 antibody
    • p100 antibody
    • Preferentially expressed in colorectal cancer antibody
    • Protein fls353 antibody
    • REPP 86 antibody
    • repp86 antibody
    • Restricted expression proliferation associated protein 100 antibody
    • Restricted expression proliferation-associated protein 100 antibody
    • Targeting protein for Xklp2 antibody
    • TPX 2 antibody
    • TPX2 antibody
    • TPX2 microtubule associated homolog antibody
    • TPX2 microtubule associated protein homolog antibody
    • TPX2 microtubule nucleation factor antibody
    • TPX2_HUMAN antibody
    see all

Images

  • ab115628, at 10 µg/ml, staining TPX2 in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Following primary incubation, slides were incubated with biotinylated goat anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115628 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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