Overview

  • Product nameAnti-TPX2 antibody
    See all TPX2 primary antibodies
  • Description
    Rabbit polyclonal to TPX2
  • Tested applicationsSuitable for: IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region between residues 150 and 200 of Human TPX2 (Swiss-Prot Q9ULW0).

  • Positive control
    • Human Breast Carcinoma, Non-Small Cell Lung Cancer and Lung Adenocarcinoma tissue. K562 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC
  • Application notesICC: 1/250.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSpindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
    • Tissue specificityExpressed in lung carcinoma cell lines but not in normal lung tissues.
    • Sequence similaritiesBelongs to the TPX2 family.
    • Developmental stageExclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • C20ORF1 antibody
      • C20orf2 antibody
      • Chromosome 20 Open Reading Frame 1 antibody
      • Differentially expressed in cancerous and non-cancerous lung cells 2 antibody
      • Differentially expressed in cancerous and noncancerous lung cells 2 antibody
      • Differentially expressed in lung cells 2 antibody
      • Differentially expressed in lung cells antibody
      • DIL 2 antibody
      • DIL-2 antibody
      • DIL2 antibody
      • FLS353 antibody
      • GD:C20orf1 antibody
      • HCA 519 antibody
      • HCA519 antibody
      • HCTP 4 antibody
      • HCTP4 antibody
      • Hepatocellular carcinoma associated antigen 519 antibody
      • Hepatocellular carcinoma-associated antigen 519 antibody
      • p100 antibody
      • Preferentially expressed in colorectal cancer antibody
      • Protein fls353 antibody
      • REPP 86 antibody
      • repp86 antibody
      • Restricted expression proliferation associated protein 100 antibody
      • Restricted expression proliferation-associated protein 100 antibody
      • Targeting protein for Xklp2 antibody
      • TPX 2 antibody
      • TPX2 antibody
      • TPX2 microtubule associated homolog antibody
      • TPX2 microtubule associated protein homolog antibody
      • TPX2_HUMAN antibody
      see all

    Anti-TPX2 antibody images

    • ab87324, at 1/250 dilution, staining TPX2 in formalin-fixed, paraffin-embedded Human lung adenocarcinoma by Immunohistochemistry using DAB staining.
    • ab87324, at 1/250 dilution, staining TPX2 in a formalin-fixed, paraffin-embedded Human K562 cell section by Immunocytochemistry using DAB staining.

    References for Anti-TPX2 antibody (ab87324)

    ab87324 has not yet been referenced specifically in any publications.

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