Overview

  • Product nameAnti-TR2-11 antibody [449C1a]
    See all TR2-11 primary antibodies
  • Description
    Mouse monoclonal [449C1a] to TR2-11
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant N terminal fragment of Human TR2-11

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab58648 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number449C1a
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 33 kDa (the immunising fragment) (predicted molecular weight: 53 kDa for full length protein).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOrphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Post-translational
      modifications
      Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression.
      Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB.
    • Cellular localizationNucleus. Nucleus > PML body. Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • NR2C 1 antibody
      • NR2C1 antibody
      • NR2C1_HUMAN antibody
      • NUCLEAR HORMONE RECEPTOR TR2 antibody
      • Nuclear receptor subfamily 2 group C member 1 antibody
      • Orphan nuclear receptor TR2 antibody
      • Testicular receptor 2 antibody
      • TR 2 antibody
      • TR2 antibody
      see all

    Anti-TR2-11 antibody [449C1a] images

    References for Anti-TR2-11 antibody [449C1a] (ab58648)

    ab58648 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"