Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

Overview

  • Product name
    Anti-TRA-1-60 (R) antibody [TRA-1-60]
  • Description
    Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IP, ICC, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.

  • General notes
    The myeloma was fused with a spleen from a Balb/c mouse.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Purity
    Tissue culture supernatant
  • Purification notes
    Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • Clonality
    Monoclonal
  • Clone number
    TRA-1-60
  • Myeloma
    P3x66Ag.8-Sp2/0
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20674000
Flow Cyt Use a concentration of 10 - 20 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    The monoclonal antibody TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The core protein recognised by TRA-1-60 has not been identified but the antibody is thought to recognise carbohydrate epitopes associated with a high molecular weight proteoglycan. The TRA antibodies are named after the Battle of Trafalgar; TRA does not stand for "Tumor Rejection Antigen" as is believed by some.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • TRA 1 60 antibody

Anti-TRA-1-60 (R) antibody [TRA-1-60] images

  • ab16288 staining TRA-160 (R) in HUES7 cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C.  Samples were incubated with primary antibody, dilution 1/500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.

    See Abreview

  • All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution

    Predicted band size : 235 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
  • ab16288 staining TRA-1-60 (R) in Human H9 cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100. Cells were incubated with the primary antibody (1/1000) for 1 hour at 4°C. Gating Strategy: Unstained H9 cells.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1/500 dilution. 

    See Abreview

References for Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

This product has been referenced in:
  • Eid W & Abdel-Rehim W Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A. Biol Chem N/A:N/A (2016). Read more (PubMed: 27343473) »
  • Howard-Jones RA  et al. Integration-Free Reprogramming of Lamina Propria Progenitor Cells. J Dent Res 95:882-8 (2016). Read more (PubMed: 26994108) »

See all 25 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human iPSC cells)
Permeabilization
Yes - 0.5% TX100
Specification
human iPSC cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 23 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cell)
Permeabilization
No
Specification
embryonic stem cell
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Kate Hawkins

Verified customer

Submitted May 17 2016

Application
Flow Cytometry
Sample
Human Cell (H9 hESCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Fibroblasts (white)
Specification
H9 hESCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (H9 human)
Specification
H9 human
Gating Strategy
Unstained H9 cells
Permeabilization
Yes - 0.2% triton x-100
Username

Abcam user community

Verified customer

Submitted Aug 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (H9 human)
Specification
H9 human
Permeabilization
Yes - 0.03% TritonX-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (H9 embryonic stem cells)
Specification
H9 embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 10 2013

Thank you for contacting us. I have checked the WB image information and indeed the figure and the legend information are contradicting. Based on the information that we have in the system, it seems that the WB Lane information was mixed-up and I s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Embryonic Stem Cell, HUES7)
Specification
Embryonic Stem Cell, HUES7
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Ms. Fiona Lewis

Verified customer

Submitted Aug 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cells)
Specification
embryonic stem cells
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Maria Keramari

Verified customer

Submitted Dec 06 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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