Overview

  • Product nameAnti-TRA-1-81 antibody [TRA-1-81]
  • Description
    Mouse monoclonal [TRA-1-81] to TRA-1-81
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ICC, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Does not react with: Mouse
  • Immunogen

    Human embryonal carcinoma cell line 2102Ep cl.2A6.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.5
  • PurityTissue culture supernatant
  • Purification notesTissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • ClonalityMonoclonal
  • Clone numberTRA-1-81
  • MyelomaP3x66Ag.8-Sp2/0
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceThe Podocalyxin gene encodes a member of the sialomucin protein family, and the encoded protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: playing a role in hematopoetic cell differentiation, binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements and being expressed in vascular endothelium cells and binding to L-selectin.
  • Cellular localizationMembrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • Gp200 antibody
    • PC antibody
    • PCLP antibody
    • PCLP1 antibody

Anti-TRA-1-81 antibody [TRA-1-81] images

  • Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg

    Secondary
    Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 200-400 kDa


    Exposure time : 3 minutes
  • ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.

    DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.

    See Abreview

  • ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.

    See Abreview

References for Anti-TRA-1-81 antibody [TRA-1-81] (ab16289)

This product has been referenced in:
  • Howard-Jones RA  et al. Integration-Free Reprogramming of Lamina Propria Progenitor Cells. J Dent Res 95:882-8 (2016). Read more (PubMed: 26994108) »
  • Intawicha P  et al. Derivation and characterization of putative embryonic stem cells from cloned rabbit embryos. Theriogenology 86:1799-810 (2016). Read more (PubMed: 27395085) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Embryonic stem cell, HUES7)
Specification Embryonic stem cell, HUES7
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Ms. Fiona Lewis

Verified customer

Submitted Oct 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (embryonic stem cells)
Specification embryonic stem cells
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Maria Keramari

Verified customer

Submitted Dec 06 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"