Overview

  • Product nameAnti-TRA2B antibody
    See all TRA2B primary antibodies
  • Description
    Rabbit polyclonal to TRA2B
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: ADVSIVYDQQ SRRSRGFAFV YFENVDDAKE AKERANGMEL DGRRIRVDFS, corresponding to internal sequence amino acids 145-194 of Human TRA2B

  • Positive control
    • 293T Cell Lysate (for WB applications) and Skeletal muscle cells (for Immunohistochemistry)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-TRA2B antibody images

  • Anti-TRA2B antibody (ab50846) at 5 µg/ml (in 5% skim milk / PBS buffer) + 239T Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/ 50,000 - 100,000 at 50000

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Gel Concentration: 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling TRA2B with ab50846 at 4-8µg/ml. Arrows indicate positively stained human skeletal muscle cells. Magnification: 400X.

References for Anti-TRA2B antibody (ab50846)

This product has been referenced in:
  • Erkelenz S  et al. Tra2-mediated recognition of HIV-1 5' splice site D3 as a key factor in the processing of vpr mRNA. J Virol 87:2721-34 (2013). Read more (PubMed: 23255807) »
  • Uren PJ  et al. Site identification in high-throughput RNA-protein interaction data. Bioinformatics 28:3013-20 (2012). IP . Read more (PubMed: 23024010) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab50846.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"