Overview

  • Product name
  • Description
    Rabbit polyclonal to TRA2B
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: ADVSIVYDQQ SRRSRGFAFV YFENVDDAKE AKERANGMEL DGRRIRVDFS, corresponding to internal sequence amino acids 145-194 of Human TRA2B

  • Positive control
    • 293T Cell Lysate (for WB applications) and Skeletal muscle cells (for Immunohistochemistry)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • Anti-TRA2B antibody (ab50846) at 5 µg/ml (in 5% skim milk / PBS buffer) + 239T Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/ 50,000 - 100,000 at 50000

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Gel Concentration: 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling TRA2B with ab50846 at 4-8µg/ml. Arrows indicate positively stained human skeletal muscle cells. Magnification: 400X.

References

This product has been referenced in:
  • Erkelenz S  et al. Tra2-mediated recognition of HIV-1 5' splice site D3 as a key factor in the processing of vpr mRNA. J Virol 87:2721-34 (2013). Read more (PubMed: 23255807) »
  • Uren PJ  et al. Site identification in high-throughput RNA-protein interaction data. Bioinformatics 28:3013-20 (2012). IP . Read more (PubMed: 23024010) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50846.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up