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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Use at an assay dependent concentration. PubMed: 22437831
Function Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Tissue specificity Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.
Sequence similarities Belongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain.
Post-translational modifications Phosphorylated in the RS domains. Dimethylation at Arg-241 is probably asymmetric.
Cellular localization Nucleus.
Information by UniProt
arginine/serine-rich 10 antibody
Arginine/serine-rich splicing factor 10 antibody
Anti-TRA2B antibody images
Western blot - TRA2B antibody (ab66901)
All lanes : Anti-TRA2B antibody (ab66901) at 1.25 µg/ml (in 5% skim milk / PBS buffer) Lane 1 : Molecular weight markers Lane 2 : Jurkat cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 32 kDa Observed band size : 40 kDa ( why is the actual band size different from the predicted?) Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TRA2B antibody(ab66901)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TRA2B with ab66901 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
References for Anti-TRA2B antibody (ab66901)
This product has been referenced in:
et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016).
Read more (PubMed: 27317697) »
et al. Tra2ß Protein Is Required for Tissue-specific Splicing of a Smooth Muscle Myosin Phosphatase Targeting Subunit Alternative Exon. J Biol Chem 287:16575-85 (2012).
Read more (PubMed: 22437831) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"