Recombinant Anti-TRA2B/SFRS10 antibody [EPR11831] (ab171082)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11831] to TRA2B/SFRS10
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TRA2B/SFRS10 antibody [EPR11831]
See all TRA2B/SFRS10 primary antibodies -
Description
Rabbit monoclonal [EPR11831] to TRA2B/SFRS10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, A431 and HeLa whole cell lysate (ab150035); Human heart and pancreas tissues; A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: Glycerol (glycerin, glycerine), BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11831 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. -
Tissue specificity
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6434 Human
- Entrez Gene: 20462 Mouse
- Entrez Gene: 117259 Rat
- Omim: 602719 Human
- SwissProt: P62995 Human
- SwissProt: P62996 Mouse
- SwissProt: P62997 Rat
- Unigene: 533122 Human
see all -
Alternative names
- arginine/serine-rich 10 antibody
- Arginine/serine-rich splicing factor 10 antibody
- hTRA2-beta antibody
see all
Images
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All lanes : Anti-TRA2B/SFRS10 antibody [EPR11831] (ab171082) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling TRA2B/SFRS10 with ab171082 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TRA2B/SFRS10 with ab171082 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of A431 cells labeling TRA2B/SFRS10 with ab171082 at 1/50 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab171082 has not yet been referenced specifically in any publications.