The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/40 - 1/80.
High temperature antigen unmasking technique. 60 minutes primary antibody incubation at 25oC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAdapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
Tissue specificityFound in all examined tissues.
Sequence similaritiesContains 1 death domain.
DomainRequires the intact DEATH domain to associate with TNFRSF1A/TNFR1.