Synthetic peptide within Human TRADD aa 133-159 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q15628
Our Abpromise guarantee covers the use of ab170371 in the following tested applications.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 34 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
Flow cytometric analysis of HeLa cells (right histogram) labeling TRADD with ab170371 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast cancer tissue labeling TRADD with ab170371 at a 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescent analysis of HeLa cells labeling TRADD with ab170371 at a 1/10 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
ab170371 has not yet been referenced specifically in any publications.