Overview

  • Product name
  • Description
    Rabbit polyclonal to TRADD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human TRADD aa 133-159 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q15628

  • Positive control
    • HeLa cell lysate; Human breast carcinoma tissue; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab170371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function
    Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
  • Tissue specificity
    Found in all examined tissues.
  • Sequence similarities
    Contains 1 death domain.
  • Domain
    Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9130005N23Rik antibody
    • AA930854 antibody
    • TNFR1 associated DEATH domain protein antibody
    • TNFR1-associated DEATH domain protein antibody
    • TNFRSF1A associated via death domain antibody
    • TNFRSF1A-associated via death domain antibody
    • tradd antibody
    • TRADD_HUMAN antibody
    • Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody
    • Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
    see all

Images

  • Anti-TRADD antibody (ab170371) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size: 34 kDa

  • Flow cytometric analysis of HeLa cells (right histogram) labeling TRADD with ab170371 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast cancer tissue labeling TRADD with ab170371 at a 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Immunofluorescent analysis of HeLa cells labeling TRADD with ab170371 at a 1/10 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

References

ab170371 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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