Overview

  • Product name
    Anti-TRADD antibody [EPR3604]
    See all TRADD primary antibodies
  • Description
    Rabbit monoclonal [EPR3604] to TRADD
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to region within Human TRADD.

  • Positive control
    • Jurkat, MCF7, HeLa and K562 cell lysates Human breast carcinoma tissue HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/10.
IHC-P 1/100. Perform antigen retrieval before commencing with IHC staining protocol.
ICC 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
    • Tissue specificity
      Found in all examined tissues.
    • Sequence similarities
      Contains 1 death domain.
    • Domain
      Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • 9130005N23Rik antibody
      • AA930854 antibody
      • TNFR1 associated DEATH domain protein antibody
      • TNFR1-associated DEATH domain protein antibody
      • TNFRSF1A associated via death domain antibody
      • TNFRSF1A-associated via death domain antibody
      • tradd antibody
      • TRADD_HUMAN antibody
      • Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody
      • Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
      see all

    Images

    • All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/500 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • ab110644, at 1/100, staining TRADD in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
    • ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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