Overview

  • Product name
  • Description
    Rabbit polyclonal to TRAF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-228 of Human TRAF1 (Uniprot ID Q13077)

  • Positive control
    • Raji cell lysate; Human SW480 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Function
    Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
  • Sequence similarities
    Contains 1 MATH domain.
  • Domain
    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling.
  • Post-translational
    modifications
    Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4732496E14Rik antibody
    • EBI6 antibody
    • EBV induced protein 6 antibody
    • Epstein-Barr virus-induced protein 6 antibody
    • MGC:10353 antibody
    • Tnf receptor associated factor 1 antibody
    • TNF receptor-associated factor 1 antibody
    • TRAF 1 antibody
    • TRAF1 antibody
    • TRAF1_HUMAN antibody
    see all

Images

  • Anti-TRAF1 antibody (ab155268) at 1/3000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SW480 xenograft tissue, labeling TRAF1 using ab155268 at a 1/100 dilution.

References

ab155268 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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