Our Abpromise guarantee covers the use of ab37119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).


  • FunctionRegulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    The RING-type zinc finger domain is essential for E3 ubiquitin-protein ligase activity. It is not essential for the stabilization of BIRC2, or for the ubiquitination of RIPK1 in response to TNFR1 signaling.
  • Post-translational
    Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.
    Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase TRAF2 antibody
    • MGC:45012 antibody
    • OTTHUMP00000022625 antibody
    • OTTHUMP00000064745 antibody
    • TNF receptor associated factor 2 antibody
    • TNF receptor-associated factor 2 antibody
    • TNF receptor-associated protein antibody
    • TRAF 2 antibody
    • TRAF2 antibody
    • TRAF2_HUMAN antibody
    • TRAP 3 antibody
    • TRAP antibody
    • TRAP3 antibody
    • Tumor necrosis factor type 2 receptor associated protein 3 antibody
    • Tumor necrosis factor type 2 receptor-associated protein 3 antibody
    see all

Anti-TRAF2 antibody images

  • Lane 1 : Anti-TRAF2 antibody (ab37119) at 0.5 µg/ml
    Lane 2 : Anti-TRAF2 antibody (ab37119) at 1 µg/ml
    Lane 3 : Anti-TRAF2 antibody (ab37119) at 2 µg/ml

    Lane 1 : Human liver cell lysate
    Lane 2 : Human liver cell lysate
    Lane 3 : Human liver cell lysate

    Lysates/proteins at 15 µg per lane.

    goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    The arrow next to the image points to the TRAF2 band.
  • ab37119 at 2.5µg/ml staining TRAF2 in human liver tissue by IHC

References for Anti-TRAF2 antibody (ab37119)

This product has been referenced in:
  • Inoue M  et al. T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation. Proc Natl Acad Sci U S A 111:5295-300 (2014). WB ; Mouse . Read more (PubMed: 24706909) »

See 1 Publication for this product

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