Overview

  • Product nameAnti-TRAF3 antibody
    See all TRAF3 primary antibodies
  • Description
    Rabbit polyclonal to TRAF3
  • SpecificityThis antibody is specific for TRAF3.
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of human TRAF3.

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab36988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionRegulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
  • Sequence similaritiesBelongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • DomainThe MATH/TRAF domain binds to receptor cytoplasmic domains.
    The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing.
  • Post-translational
    modifications
    Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.
  • Cellular localizationCytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP 1 antibody
    • CAP-1 antibody
    • CAP1 antibody
    • CD40 associated protein 1 antibody
    • CD40 binding protein antibody
    • CD40 bp antibody
    • CD40 receptor associated factor 1 antibody
    • CD40 receptor-associated factor 1 antibody
    • CD40-binding protein antibody
    • CD40BP antibody
    • CRAF 1 antibody
    • CRAF1 antibody
    • IIAE5 antibody
    • LAP 1 antibody
    • LAP1 antibody
    • LMP1 associated protein antibody
    • LMP1-associated protein 1 antibody
    • MIPT3 antibody
    • TNF receptor associated factor 3 antibody
    • TNF receptor-associated factor 3 antibody
    • TRAF 3 antibody
    • Traf3 antibody
    • TRAF3_HUMAN antibody
    see all

Anti-TRAF3 antibody images

  • Lane 1 : Anti-TRAF3 antibody (ab36988) at 2 µg/ml
    Lane 2 : Anti-TRAF3 antibody (ab36988) at 4 µg/ml

    Lane 1 : Hela cell lysate.
    Lane 2 : Hela cell lysate.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
  • ab36988 at 10µg/ml staining TRAF3 in Hela cells by ICC/IF

References for Anti-TRAF3 antibody (ab36988)

This product has been referenced in:
  • Mankouri J  et al. Optineurin negatively regulates the induction of IFNbeta in response to RNA virus infection. PLoS Pathog 6:e1000778 (2010). Read more (PubMed: 20174559) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - other (cortex)
Total protein in input 200 µg
Specification cortex
Immuno-precipitation step Protein G
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Verified customer

Submitted May 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"