Overview

  • Product nameAnti-TRAF3IP3 antibody
    See all TRAF3IP3 primary antibodies
  • Description
    Rabbit polyclonal to TRAF3IP3
  • Tested applicationsSuitable for: IP, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized peptide derived from an internal sequence of human TRAF3IP3.

  • Positive control
    • Extracts from 293 cells; HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA 1/20000.
ICC/IF Use at an assay dependent dilution.

Target

Anti-TRAF3IP3 antibody images

  • All lanes : Anti-TRAF3IP3 antibody (ab69623) at 1/500 dilution

    Lane 1 : Extracts from 293 cells with no immunizing peptide
    Lane 2 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells, using ab69623 at a dilution of 1/500. Left hand image - without immunizing peptide; right hand image - with immunizing peptide.

References for Anti-TRAF3IP3 antibody (ab69623)

ab69623 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - other (cortex)
Total protein in input 200 µg
Specification cortex
Immuno-precipitation step Protein G
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Submitted May 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"