Overview

  • Product nameAnti-TRAF4 antibody
    See all TRAF4 primary antibodies
  • Description
    Rabbit polyclonal to TRAF4
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (PGLAKPQHVT ETFHPDPNWK NFQKPGTWRG SLDESSLGFG YPKFISHQDI) of human TRAF4 (NP_004286).

  • Positive control
    • Hela cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAdapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
  • Tissue specificityExpressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
  • Sequence similaritiesBelongs to the TNF receptor-associated factor family. B subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 3 TRAF-type zinc fingers.
  • DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CART 1 antibody
    • CART1 antibody
    • cysteine rich domain associated with ring and TRAF domain antibody
    • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
    • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
    • Malignant 62 antibody
    • Metastatic lymph node gene 62 protein antibody
    • MLN 62 antibody
    • MLN62 antibody
    • RING finger protein 83 antibody
    • RNF 83 antibody
    • RNF83 antibody
    • TNF receptor associated factor 4 antibody
    • TNF receptor-associated factor 4 antibody
    • TRAF 4 antibody
    • Traf4 antibody
    • TRAF4_HUMAN antibody
    • tumor necrosis receptor-associated factor 4A antibody
    see all

Anti-TRAF4 antibody images

  • Anti-TRAF4 antibody (ab83227) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 27 kDa,>90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TRAF4 antibody (ab83227)

ab83227 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"