Anti-TRAF4 antibody - C-terminal (ab190986)
Key features and details
- Rabbit polyclonal to TRAF4 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRAF4 antibody - C-terminal
See all TRAF4 primary antibodies -
Description
Rabbit polyclonal to TRAF4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human TRAF4 aa 400 to the C-terminus (C terminal).
Database link: Q9BUZ4 -
Positive control
- Rat thymus, Hela, Jurkat, Hepa lysates. Rat lymphonodus tissue and human lung cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 54 kDa.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 54 kDa. |
Target
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Function
Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. -
Tissue specificity
Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. -
Sequence similarities
Belongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 9618 Human
- Entrez Gene: 22032 Mouse
- Entrez Gene: 303285 Rat
- Omim: 602464 Human
- SwissProt: Q9BUZ4 Human
- SwissProt: Q61382 Mouse
- Unigene: 8375 Human
- Unigene: 390418 Mouse
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Alternative names
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
see all
Images
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All lanes : Anti-TRAF4 antibody - C-terminal (ab190986) at 0.5 µg/ml
Lane 1 : Rat thymus lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : Hepa lysate
Predicted band size: 54 kDa -
Immunohistochemical analysis of formalin fixed paraffin embedded human lung cancer tissue labeling TRAF4 with ab190986 at 1 µg/ml.
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Immunohistochemical analysis of formalin fixed paraffin embedded rat lymphonodus tissue labeling TRAF4 with ab190986 at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab190986 has been referenced in 2 publications.
- Kang Q et al. Role of the overexpression of TRAF4 in predicting the prognosis of intrahepatic cholangiocarcinoma. Int J Oncol 53:286-296 (2018). PubMed: 29749456
- Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . PubMed: 27842582