Overview

  • Product name
    Anti-TRAF4 antibody - C-terminal
    See all TRAF4 primary antibodies
  • Description
    Rabbit polyclonal to TRAF4 - C-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRAF4 aa 437-454 (C terminal).
    Sequence:

    PKFISHQDIRKRNYVRDD


    Database link: Q9BUZ4

  • Positive control
    • Rat thymus, Hela, Jurkat, Hepa lysates. Rat lymphonodus tissue and human lung cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab190986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 54 kDa.

Target

  • Function
    Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
  • Tissue specificity
    Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
  • Sequence similarities
    Belongs to the TNF receptor-associated factor family. B subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 3 TRAF-type zinc fingers.
  • Domain
    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CART 1 antibody
    • CART1 antibody
    • cysteine rich domain associated with ring and TRAF domain antibody
    • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
    • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
    • Malignant 62 antibody
    • Metastatic lymph node gene 62 protein antibody
    • MLN 62 antibody
    • MLN62 antibody
    • RING finger protein 83 antibody
    • RNF 83 antibody
    • RNF83 antibody
    • TNF receptor associated factor 4 antibody
    • TNF receptor-associated factor 4 antibody
    • TRAF 4 antibody
    • Traf4 antibody
    • TRAF4_HUMAN antibody
    • tumor necrosis receptor-associated factor 4A antibody
    see all

Images

  • All lanes : Anti-TRAF4 antibody - C-terminal (ab190986) at 0.5 µg/ml

    Lane 1 : Rat thymus lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate
    Lane 4 : Hepa lysate


    Predicted band size : 54 kDa
  • Immunohistochemical analysis of formalin fixed paraffin embedded human lung cancer tissue labeling TRAF4 with ab190986 at 1 µg/ml.

  • Immunohistochemical analysis of  formalin fixed paraffin embedded rat lymphonodus tissue labeling TRAF4 with ab190986 at 1 µg/ml.

References

This product has been referenced in:
  • Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . Read more (PubMed: 27842582) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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