Overview

  • Product nameAnti-TRAF4 antibody [EPR1729]
    See all TRAF4 primary antibodies
  • Description
    Rabbit monoclonal [EPR1729] to TRAF4
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Jurkat, HeLa and SKBR-3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionAdapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
    • Tissue specificityExpressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
    • Sequence similaritiesBelongs to the TNF receptor-associated factor family. B subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 3 TRAF-type zinc fingers.
    • DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
      The MATH/TRAF domain binds to receptor cytoplasmic domains.
    • Post-translational
      modifications
      Polyubiquitinated, leading to its proteasomal degradation.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • CART 1 antibody
      • CART1 antibody
      • cysteine rich domain associated with ring and TRAF domain antibody
      • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
      • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
      • Malignant 62 antibody
      • Metastatic lymph node gene 62 protein antibody
      • MLN 62 antibody
      • MLN62 antibody
      • RING finger protein 83 antibody
      • RNF 83 antibody
      • RNF83 antibody
      • TNF receptor associated factor 4 antibody
      • TNF receptor-associated factor 4 antibody
      • TRAF 4 antibody
      • Traf4 antibody
      • TRAF4_HUMAN antibody
      • tumor necrosis receptor-associated factor 4A antibody
      see all

    Anti-TRAF4 antibody [EPR1729] images

    • Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 54 kDa

    References for Anti-TRAF4 antibody [EPR1729] (ab108991)

    This product has been referenced in:
    • Zepp JA  et al. Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes. J Immunol 189:33-7 (2012). Read more (PubMed: 22649194) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (LnCaP cells)
    Loading amount 50 µg
    Specification LnCaP cells
    Gel Running Conditions Reduced Denaturing (5-15% gradient gel)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"