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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/1000 - 1/2500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
chromosome 15 open reading frame 23 antibody
Anti-TRAF4AF1 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-TRAF4AF1 antibody (ab122769)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab122769 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF4AF1 antibody (ab122769)
ab122769, at 1/50 dilution, staining TRAF4AF1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Western blot - Anti-TRAF4AF1 antibody (ab122769)
All lanes : Anti-TRAF4AF1 antibody (ab122769) at 1/250 dilution Lane 1 : RT-4 lysate Lane 2 : U251-MG lysate Lane 3 : Human plasma Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate
References for Anti-TRAF4AF1 antibody (ab122769)
This product has been referenced in:
et al. Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19). PLoS One 9:e92712 (2014).
Read more (PubMed: 24718257) »
et al. Recurrent point mutations in the kinetochore gene KNSTRN in cutaneous squamous cell carcinoma. Nat Genet 46:1060-2 (2014).
Read more (PubMed: 25194279) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"