For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to TRAF4AF1
TVYSLQPPSA LSGGQPADTQ TRATSKSLLP VRSKEVDVSK QLHSGGPEND VTKITKLRRE NGQMKATDTA TRRNVRKGYK PLSKQ, corresponding to amino acids 85-169 of Human TRAF4AF1
Human kidney tissue; RT-4 and U251-MG lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/1000 - 1/2500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
chromosome 15 open reading frame 23 antibody
Immunocytochemistry/ Immunofluorescence - Anti-TRAF4AF1 antibody (ab122769)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab122769 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF4AF1 antibody (ab122769)
ab122769, at 1/50 dilution, staining TRAF4AF1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Western blot - Anti-TRAF4AF1 antibody (ab122769)
All lanes : Anti-TRAF4AF1 antibody (ab122769) at 1/250 dilution Lane 1 : RT-4 lysate Lane 2 : U251-MG lysate Lane 3 : Human plasma Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"