Overview

  • Product name
    Anti-TRAF4AF1 antibody
  • Description
    Rabbit polyclonal to TRAF4AF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    TVYSLQPPSA LSGGQPADTQ TRATSKSLLP VRSKEVDVSK QLHSGGPEND VTKITKLRRE NGQMKATDTA TRRNVRKGYK PLSKQ, corresponding to amino acids 85-169 of Human TRAF4AF1

  • Positive control
    • Human kidney tissue; RT-4 and U251-MG lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/1000 - 1/2500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KNSTRN antibody
    • C15orf23 antibody
    • chromosome 15 open reading frame 23 antibody
    • HSD11 antibody
    • Kinastrin antibody
    • Kinetochore-localized astrin-binding protein antibody
    • Kinetochore-localized astrin/SPAG5-binding protein antibody
    • Putative TRAF4-associated factor 1 antibody
    • SKAP antibody
    • small kinetochore associated protein antibody
    • T4AF1_HUMAN antibody
    • TRAF4 associated factor 1 antibody
    • TRAF4-associated factor 1 antibody
    • Traf4af1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab122769 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122769, at 1/50 dilution, staining TRAF4AF1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • All lanes : Anti-TRAF4AF1 antibody (ab122769) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U251-MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References

This product has been referenced in:
  • Lu S  et al. Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19). PLoS One 9:e92712 (2014). WB ; Human . Read more (PubMed: 24718257) »
  • Lee CS  et al. Recurrent point mutations in the kinetochore gene KNSTRN in cutaneous squamous cell carcinoma. Nat Genet 46:1060-2 (2014). Read more (PubMed: 25194279) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122769.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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