The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa).
Use at an assay dependent concentration.
1/50 - 1/200.
FunctionAdapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis.
Tissue specificityExpressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.
Sequence similaritiesBelongs to the TNF receptor-associated factor family. A subfamily. Contains 1 MATH domain. Contains 1 RING-type zinc finger. Contains 2 TRAF-type zinc fingers.
DomainThe MATH/TRAF domain binds to receptor cytoplasmic domains.
All lanes : Anti-TRAF5 antibody (ab47539) at 1/2000 dilution
Lane 1 : Normal human testis tissue Lane 2 : Normal human kidney tissue Lane 3 : Normal human pancreas tissue Lane 4 : Normal human liver tissue Lane 5 : Normal human prostate tissue Lane 6 : Normal human breast tissue Lane 7 : Normal human colon tissue Lane 8 : Normal human thymus tissue Lane 9 : Normal human lymph node tissue Lane 10 : Normal human spleen tissue Lane 11 : Normal human lung tissue Lane 12 : Normal human aorta tissue Lane 13 : Normal human epidermis tissue
Predicted band size : 64 kDa Observed band size : 64 kDa