Anti-TRAIL antibody [55B709.3] (ab12124)

Overview

  • Product nameAnti-TRAIL antibody [55B709.3]See all TRAIL primary antibodies ...
  • Description
    Mouse monoclonal [55B709.3] to TRAIL
  • Specificityab12124 reacts with human TRAIL.
  • Tested applicationsWB, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 17-35 of Human TRAIL.

  • Positive control
    • Jurkat whole-cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab12124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 33.7 kDa). For optimal results primary antibody incubation should be performed at room temperature.
Sandwich ELISA Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TRAIL (ab9959). For sandwich ELISA, use this antibody as Capture at 0.2µg/ml with ab9959 as Detection.

Target

  • FunctionCytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • Tissue specificityWidespread; most predominant in spleen, lung and prostate.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P50591 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Apo 2 ligand antibody
    • Apo 2L antibody
    • Apo-2 ligand antibody
    • Apo-2L antibody
    • APO2L antibody
    • CD 253 antibody
    • CD253 antibody
    • CD253 antigen antibody
    • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
    • Protein TRAIL antibody
    • TL 2 antibody
    • TL2 antibody
    • TNF Related Apoptosis Inducing Ligand antibody
    • TNF related apoptosis inducing ligand TRAIL antibody
    • TNF-related apoptosis-inducing ligand antibody
    • TNF10_HUMAN antibody
    • TNFSF 10 antibody
    • TNFSF10 antibody
    • TNFSF10 protein antibody
    • TRAIL antibody
    • TRAIL PEN antibody
    • Tumor necrosis factor (ligand) family member 10 antibody
    • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
    • Tumor necrosis factor ligand superfamily member 10 antibody
    see all

Anti-TRAIL antibody [55B709.3] images

  • Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1µg/ml.


  • Predicted band size : 33.7 kDa


    TRAIL detection by Western blot. The analysis of 10 µg of total cell extract from Jurkat cells with ab12124 at a 2 ug/ml dilution.

Protocols

References for Anti-TRAIL antibody [55B709.3] (ab12124)

ab12124 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am pleased to help answer your questions. 1. I am sorry to confirm that ab12124 antibody would not be suitable for detection of the ab78818 protein. As you have noticed from the different amino acid sequenc...

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Thank you for your email. I have arranged for a replacement vial of ab12124 (from a newer batch) to be sent to you free of charge. It is on order # 87585 and you should receive this around the middle of next week (it is currently out of stock in our US...

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Thank you for the details in which you have provided. I contacted our source for this antibody and they have not had any complaints regarding this antibody. The particular lot that you received worked very well with the Jurkat whole-cell extract that w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"