Anti-TRAIL antibody (ab231063)
Key features and details
- Rabbit polyclonal to TRAIL
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRAIL antibody
See all TRAIL primary antibodies -
Description
Rabbit polyclonal to TRAIL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Rat TRAIL aa 100 to the C-terminus. N-terminal His-tag and GST-tag. Expressed in E. coli.
Database link: Q8K3G0 -
Positive control
- WB: Recombinant rat TRAIL protein. MCF7 cell lysate. IHC-P: Rat kidney, intestine, stomach, ovary and testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
Tissue specificity
Widespread; most predominant in spleen, lung and prostate. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8743 Human
- Entrez Gene: 246775 Rat
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
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Alternative names
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat intestine tissue stained for TRAIL with ab231063 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-TRAIL antibody (ab231063) at 3 µg/ml + MCF7 (Human breast adenocarcinoma cell line) cell lysate
Developed using the ECL technique. -
Formalin-fixed, paraffin-embedded rat kidney tissue stained for TRAIL with ab231063 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded rat ovary tissue stained for TRAIL with ab231063 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded rat testis tissue stained for TRAIL with ab231063 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded rat stomach tissue stained for TRAIL with ab231063 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-TRAIL antibody (ab231063) at 2 µg/ml + recombinant rat TRAIL protein
Developed using the ECL technique.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab231063 has been referenced in 3 publications.
- Yang Y et al. Moderate Mechanical Stimulation Protects Rats against Osteoarthritis through the Regulation of TRAIL via the NF-?B/NLRP3 Pathway. Oxid Med Cell Longev 2020:6196398 (2020). PubMed: 32566090
- Gong H et al. Tumor necrosis factor-related apoptosis-inducing ligand inhibits the growth and aggressiveness of colon carcinoma via the exogenous apoptosis signaling pathway. Exp Ther Med 17:41-50 (2019). PubMed: 30651763
- Arnold ND et al. A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension. Nat Commun 10:5183 (2019). PubMed: 31729368