Overview

  • Product name
    trans-Retinoic acid
  • Description
    Vitamin A metabolite. Stem cell differentiator.
  • Alternative names
    • ATRA
  • Biological description
    Vitamin A metabolite. Induces apoptosis, shows antimitotic and antitumor activity. Differentiates various stem cells and neural progenitor cells.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid (ab120728), by ICC/IF. Decrease of Oct4 expression correlates with increased concentration of trans-retinoic acid, as described in literature.
    The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19857 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Karsy M  et al. All-trans retinoic acid modulates cancer stem cells of glioblastoma multiforme in an MAPK-dependent manner. Anticancer Res 30:4915-20 (2010). Read more (PubMed: 21187470) »
  • Guo J  et al. Antitumor effects of all-trans-retinoic acid on cultured human pancreatic cancer cells. J Gastroenterol Hepatol 21:443-8 (2006). Read more (PubMed: 16509872) »
  • Guo JM  et al. The effects of all-trans-retinoic acid on cell cycle and alkaline phosphatase activity in pancreatic cancer cells. Med Chem 2:457-61 (2006). Read more (PubMed: 17017984) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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