Anti-Transaldolase 1 antibody (ab187730)
Key features and details
- Rabbit polyclonal to Transaldolase 1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Transaldolase 1 antibody
See all Transaldolase 1 primary antibodies -
Description
Rabbit polyclonal to Transaldolase 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide within Human Transaldolase 1 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P37837 -
Positive control
- HeLa, Jurkat, 293, NIH 3T3 and TCMK-1 cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 37 kDa. |
Target
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Function
Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3. -
Involvement in disease
Defects in TALDO1 are the cause of transaldolase 1 deficiency (TALDO1 deficiency) [MIM:606003]. It results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris. -
Sequence similarities
Belongs to the transaldolase family. Type 1 subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6888 Human
- Entrez Gene: 21351 Mouse
- Omim: 602063 Human
- SwissProt: P37837 Human
- SwissProt: Q93092 Mouse
- Unigene: 438678 Human
- Unigene: 29182 Mouse
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Alternative names
- Dihydroxyacetone transferase antibody
- EC 2.2.1.2 antibody
- EPS8L2 antibody
see all
Images
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All lanes : Anti-Transaldolase 1 antibody (ab187730) at 0.4 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse TCMK-1 cell lysate
Lane 5 : Mouse NIH 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 37 kDa -
Immunoprecipitation of Transaldolase 1 from HeLa whole cell lysate (1 mg per lane), using ab187730 at 6 µg (lane 1) or an IgG control (lane 2). For western blot of immunoprecipitated protein, ab187730 was used at 1 µg/ml.
Detection: ECL with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab187730 has not yet been referenced specifically in any publications.