Overview

  • Product nameAnti-Transaldolase 1 antibody
    See all Transaldolase 1 primary antibodies
  • Description
    Mouse polyclonal to Transaldolase 1
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Transaldolase 1 protein (Human)

  • Positive control
    • Transaldolase 1 transfected 293T cell lysate and human kidney tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
    Detection Antibody Pair


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTransaldolase is important for the balance of metabolites in the pentose-phosphate pathway.
    • PathwayCarbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.
    • Involvement in diseaseDefects in TALDO1 are the cause of transaldolase 1 deficiency (TALDO1 deficiency) [MIM:606003]. It results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.
    • Sequence similaritiesBelongs to the transaldolase family. Type 1 subfamily.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dihydroxyacetone transferase antibody
      • EC 2.2.1.2 antibody
      • EPS8L2 antibody
      • Glycerone transferase antibody
      • TAL antibody
      • TAL H antibody
      • TALDO antibody
      • TALDO_HUMAN antibody
      • TALDO1 antibody
      • TALDOR antibody
      • TALH antibody
      • Transaldolase 1 antibody
      • Transaldolase antibody
      see all

    Anti-Transaldolase 1 antibody images

    • Anti-Transaldolase 1 antibody (ab67467) at 1/500 dilution + human kidney tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Transaldolase 1 antibody (ab67467) at 1/500 dilution

      Lane 1 : Transaldolase 1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-Transaldolase 1 antibody (ab67467)

    This product has been referenced in:
    • Kocevar N  et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . Read more (PubMed: 22468085) »
    • Alberio T  et al. Discovery and verification of panels of T-lymphocyte proteins as biomarkers of Parkinson's disease. Sci Rep 2:953 (2012). WB ; Human . Read more (PubMed: 23233872) »

    See all 3 Publications for this product

    Product Wall

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Stomach)
    Loading amount 30 µg
    Specification Stomach
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Verified customer

    Submitted Jul 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"