Anti-Transcription factor 25 antibody (ab96813)

Overview

  • Product name
    Anti-Transcription factor 25 antibody
    See all Transcription factor 25 primary antibodies
  • Description
    Rabbit polyclonal to Transcription factor 25
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 427 and 648 of Transcription factor 25 (NP_055787)

  • Positive control
    • Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab96813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function
    May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
  • Tissue specificity
    In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
  • Sequence similarities
    Belongs to the TCF25 family.
  • Domain
    The C-terminal region mediates transcriptional repression.
  • Cellular localization
    Nucleus. Some staining in the cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKSG26 antibody
    • hKIAA1049 antibody
    • Hulp1 antibody
    • KIAA1049 antibody
    • Nuclear localized protein 1 antibody
    • NULP1 antibody
    • PRO2620 antibody
    • TCF-25 antibody
    • Tcf25 antibody
    • TCF25_HUMAN antibody
    • Transcription factor 25 (basic helix loop helix) antibody
    • Transcription factor 25 antibody
    see all

Images

  • Anti-Transcription factor 25 antibody (ab96813) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 77 kDa

    7.5% SDS Page

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.

References

ab96813 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Loading amount
40 µg
Specification
HeLa cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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