Anti-Transcription factor 25 antibody (ab96813)

Overview

  • Product nameAnti-Transcription factor 25 antibody
    See all Transcription factor 25 primary antibodies
  • Description
    Rabbit polyclonal to Transcription factor 25
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 427 and 648 of Transcription factor 25 (NP_055787)

  • Positive control
    • Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
  • Tissue specificityIn the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
  • Sequence similaritiesBelongs to the TCF25 family.
  • DomainThe C-terminal region mediates transcriptional repression.
  • Cellular localizationNucleus. Some staining in the cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKSG26 antibody
    • hKIAA1049 antibody
    • Hulp1 antibody
    • KIAA1049 antibody
    • Nuclear localized protein 1 antibody
    • NULP1 antibody
    • PRO2620 antibody
    • TCF-25 antibody
    • Tcf25 antibody
    • TCF25_HUMAN antibody
    • Transcription factor 25 (basic helix loop helix) antibody
    • Transcription factor 25 antibody
    see all

Anti-Transcription factor 25 antibody images

  • Anti-Transcription factor 25 antibody (ab96813) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 77 kDa

    7.5% SDS Page

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.

References for Anti-Transcription factor 25 antibody (ab96813)

ab96813 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell line)
Loading amount 40 µg
Specification HeLa cell line
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"