Anti-TTF1 antibody (ab87726)
Key features and details
- Rabbit polyclonal to TTF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TTF1 antibody
See all TTF1 primary antibodies -
Description
Rabbit polyclonal to TTF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human TTF1 aa 1-100 (N terminal). (CAA58807.1)
Database link: Q15361 -
Positive control
- HeLa or 293T cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 103 kDa.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 103 kDa. |
Target
-
Function
Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity. -
Sequence similarities
Contains 2 Myb-like domains. -
Domain
The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 7270 Human
- Omim: 600777 Human
- SwissProt: Q15361 Human
- Unigene: 54780 Human
-
Alternative names
- RNA polymerase I termination factor antibody
- Transcription termination factor 1 antibody
- Transcription termination factor I antibody
see all
Images
-
All lanes : Anti-TTF1 antibody (ab87726) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~118 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab87726 has been referenced in 1 publication.
- Komatsu H et al. Clinical and biological significance of transcription termination factor, RNA polymerase I in human liver hepatocellular carcinoma. Oncol Rep 35:2073-80 (2016). PubMed: 26821084