Anti-Transcription termination factor 1 antibody (ab87726)

Overview

  • Product name
    Anti-Transcription termination factor 1 antibody
    See all Transcription termination factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Transcription termination factor 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Transcription termination factor 1 (CAA58807.1)

  • Positive control
    • HeLa or 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 103 kDa.

Target

  • Function
    Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
  • Sequence similarities
    Contains 2 Myb-like domains.
  • Domain
    The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • RNA polymerase I termination factor antibody
    • Transcription termination factor 1 antibody
    • Transcription termination factor I antibody
    • Transcription termination factor RNA polymerase I antibody
    • TTF 1 antibody
    • TTF-1 antibody
    • TTF-I antibody
    • Ttf1 antibody
    • TTF1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Transcription termination factor 1 antibody (ab87726) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : ~118 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References

ab87726 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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