Anti-Transferrin antibody [11D3] (HRP) (ab10208)


  • Product name
    Anti-Transferrin antibody [11D3] (HRP)
    See all Transferrin primary antibodies
  • Description
    Mouse monoclonal [11D3] to Transferrin (HRP)
  • Conjugation
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Placental transferrin receptor or transferrin.

  • Positive control
    • human brain
  • General notes
    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.



Our Abpromise guarantee covers the use of ab10208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be used as both coating and conjugate antibody when paired with recommended pair for detection of transferrin.
WB 1/500. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).


  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all


  • All lanes : Anti-Transferrin antibody [11D3] (HRP) (ab10208) at 1/500 dilution

    Lane 1 : human brain
    Lane 2 : human brain
    Lane 3 : human brain
    Lane 4 : human brain
    Lane 5 : human brain
    Lane 6 : human brain
    Lane 7 : human brain
    Lane 8 : human brain
    Lane 9 : human brain

    Lysates/proteins at 20 µg per lane.

    DAKO Cytomation Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : many <77 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom


This product has been referenced in:
  • Shakor AB  et al. Sphingomyelin Synthase 1-generated Sphingomyelin Plays an Important Role in Transferrin Trafficking and Cell Proliferation. J Biol Chem 286:36053-62 (2011). Read more (PubMed: 21856749) »
  • Grønborg M  et al. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol Cell Proteomics 5:157-71 (2006). Read more (PubMed: 16215274) »

See all 3 Publications for this product

Customer reviews and Q&As

According to the testing lab, the sELISA was performed on these conditions:

Capture Ab10212: 10 μg/ml for plate coating

Detection Ab10208: 1.5 μg/ml
Human transferrin antigen – different concentrations

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (Brain lysate)
Brain lysate
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

Thank you for your enquiry. We would recommend the typical ELISA coating dilutions which we start with: 50 uL/well of a 2 ug/mL solution. The tracer antibody dilution depends on the conjugates recommended dilution range. Hope this helps.


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