Anti-Transferrin antibody [11D3] (HRP) (ab10208)

Overview

  • Product nameAnti-Transferrin antibody [11D3] (HRP)
    See all Transferrin primary antibodies
  • Description
    Mouse monoclonal [11D3] to Transferrin (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Placental transferrin receptor or transferrin.

  • Positive control
    • human brain
  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [8B9] to Transferrin (ab10212). Can be used as both coating and conjugate antibody when paired with recommended pair for detection of transferrin.
WB 1/500. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

Target

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody [11D3] (HRP) images

  • All lanes : Anti-Transferrin antibody [11D3] (HRP) (ab10208) at 1/500 dilution

    Lane 1 : human brain
    Lane 2 : human brain
    Lane 3 : human brain
    Lane 4 : human brain
    Lane 5 : human brain
    Lane 6 : human brain
    Lane 7 : human brain
    Lane 8 : human brain
    Lane 9 : human brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    DAKO Cytomation Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : many <77 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

References for Anti-Transferrin antibody [11D3] (HRP) (ab10208)

This product has been referenced in:
  • Shakor AB  et al. Sphingomyelin Synthase 1-generated Sphingomyelin Plays an Important Role in Transferrin Trafficking and Cell Proliferation. J Biol Chem 286:36053-62 (2011). Read more (PubMed: 21856749) »
  • Grønborg M  et al. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol Cell Proteomics 5:157-71 (2006). Read more (PubMed: 16215274) »

See all 3 Publications for this product

Product Wall



According to the testing lab, the sELISA was performed on these conditions:

Capture Ab10212: 10 μg/ml for plate coating

Detection Ab10208: 1.5 μg/ml
Human transferrin antigen – different concentrations
P...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain lysate)
Specification Brain lysate
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

Thank you for your enquiry. We would recommend the typical ELISA coating dilutions which we start with: 50 uL/well of a 2 ug/mL solution. The tracer antibody dilution depends on the conjugates recommended dilution range. Hope this helps.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"