The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
1/150000 - 1/300000. Coating antigen 800ng/well
Use a concentration of 5 µg/ml.
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Expressed by the liver and secreted in plasma.
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Belongs to the transferrin family. Contains 2 transferrin-like domains.
ICC/IF image of ab66952 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66952, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab66952, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Di Luca A et al. 2D DIGE proteomic analysis of early post mortem muscle exudate highlights the importance of the stress response for improved water-holding capacity of fresh pork meat. Proteomics13:1528-44 (2013).
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