Overview

  • Product name
  • Description
    Rabbit polyclonal to Transferrin
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Dog, Human
  • Immunogen

    Human Transferrin Protein

  • Positive control
    • Rat apotransferrin protein. IF: HepG2 cell line This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab82411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/10000. Predicted molecular weight: 77 kDa.
IP Use at an assay dependent concentration.

Target

  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Images

  • Anti-Transferrin antibody (ab82411) at 1/10000 dilution + rat apotransferrin
    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ICC/IF image of ab82411 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Transferrin staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82411, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Anti-Transferrin antibody (ab82411) at 1/1000 dilution + Mouse pancreatic acinar cells at 30 µg with Milk, 12 hours, 4°C at 5 %

    Secondary
    Goat anti-rabbit IgG (HRP conjugated) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

References

This product has been referenced in:
  • Zauberman A  et al. Host Iron Nutritional Immunity Induced by a Live Yersinia pestis Vaccine Strain Is Associated with Immediate Protection against Plague. Front Cell Infect Microbiol 7:277 (2017). Read more (PubMed: 28680860) »
  • Chan B  et al. A mouse model of a human congenital disorder of glycosylation caused by loss of PMM2. Hum Mol Genet 25:2182-2193 (2016). WB . Read more (PubMed: 27053713) »

See all 7 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Serum (Mouse serum)
Gel Running Conditions
Non-reduced Denaturing (4-20 % gradient gel)
Loading amount
50 µg
Specification
Mouse serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 11 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.2% triton x 100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse pancreatic acinar cells)
Loading amount
30 µg
Specification
Mouse pancreatic acinar cells
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20 %)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

Thank you for calling Abcam earlier today.
I did a little more searching and I found that we already have the blocking peptide for ab16051 in stock, it is ab16377:
http://www.abcam.com/beta-catenin-peptide-ab16377.html
As for ab82411, I ...

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Blocking buffer has been used as diluent. This particular antibody was tested at 1:2000 using 5%NFD milk/1xPBS/0.05%Tween20. The WB protocol followed is overnight blocking at 4C and 1 hour incubations at room temp with the primary and then with the s...

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