Overview

  • Product nameAnti-Transferrin antibody
    See all Transferrin primary antibodies
  • Description
    Sheep polyclonal to Transferrin
  • SpecificityThis product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity is confirmed by double diffusion (Ouchterlony) vs human serum and a known anti-human transferrin.
  • Tested applicationsSuitable for: WB, Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Human
    Does not react with: Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Human holo-transferrin purified from serum and shown to be homogeneous by SDS-PAGE.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Double Immunodiffusion
RID
Immunoelectrophoresis
Rocket Immunoelectrophoresis
  • Application notesWB: Use at an assay dependent dilution (from PMID 17611631)
    RID and Rocket IEP: 1.5µL antiserum/cm² gel vs 5µL 1/5 – 1/50 dilution human serum
    Double diffusion: 10µL antiserum vs 5µL serum
    IEP: 100µL antiserum vs 5µL serum.

    Optimal dilutions should be determined by the individual laboratory
    The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
    Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
  • Target

    • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
    • Sequence similaritiesBelongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    References for Anti-Transferrin antibody (ab9033)

    This product has been referenced in:
    • Carberry S  et al. Comparative proteomic analysis of the contractile-protein-depleted fraction from normal versus dystrophic skeletal muscle. Anal Biochem N/A:N/A (2013). Read more (PubMed: 23954569) »
    • O'Connell K  et al. Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle. Int J Mol Med 20:145-53 (2007). WB ; Rat . Read more (PubMed: 17611631) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"