Antiserum is prepared by immunisation of sheep with Human Holo-Transferrin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents.
An immunoglobulin fraction is then produced.
The titre is adjusted so that inter-batch variation is within 10%.
The product is 0.2µm filtered.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution (from PMID 17611631)
RID and Rocket IEP: 1.5µL antiserum/cm² gel vs 5µL 1/5 – 1/50 dilution human serum
Double diffusion: 10µL antiserum vs 5µL serum
IEP: 100µL antiserum vs 5µL serum.
Optimal dilutions should be determined by the individual laboratory
The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded.
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Expressed by the liver and secreted in plasma.
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Belongs to the transferrin family. Contains 2 transferrin-like domains.
Murphy S et al. Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med39:1357-1370 (2017).
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Carberry S et al. Comparative proteomic analysis of the contractile-protein-depleted fraction from normal versus dystrophic skeletal muscle. Anal BiochemN/A:N/A (2013).
Read more (PubMed: 23954569) »