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Our Abpromise guarantee covers the use of ab9538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [HTF-14] to Transferrin (ab769). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab769 as Capture.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/100. ABC method.|
|IHC-Fr||1/50 - 1/100. ABC method.|