Anti-Transferrin antibody (Biotin) (ab34673)

Overview

  • Product nameAnti-Transferrin antibody (Biotin)
    See all Transferrin primary antibodies
  • Description
    Rabbit polyclonal to Transferrin (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, Dot blot, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Transferrin from Human serum.

  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab34673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
Dot blot Use at an assay dependent concentration.
ELISA 1/20000 - 1/150000.

This antibody has been assayed against 1.0µg of Transferrin in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

Target

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody (Biotin) images



  • Performed under reducing conditions.

    Predicted band size : 77 kDa

    Multiplex western blot analysis of 35 µg of human serum with 350 ng of added GST. Green bands are labelling human transferrin, with ab34673 and a donkey anti-rabbit DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a donkey anti-goat DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a donkey anti-mouse DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 30 minutes at 4°C.

  • Anti-Transferrin antibody (Biotin) (ab34673) at 1/1000 dilution + Human depleted serum with Blocking buffer

    Secondary
    Peroxidase streptavidin conjugate at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size : 77 kDa


  • Performed under reducing conditions.

    Predicted band size : 77 kDa

    Multiplex western blot analysis of human serum. Green bands are labelling human transferrin, with ab34673 and a DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 1 hour at 4°C.

References for Anti-Transferrin antibody (Biotin) (ab34673)

ab34673 has not yet been referenced specifically in any publications.

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