SpecificityPrior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum, dilution 1:10, or human transferrin. Only one precipitin arc in immunoelectrophoresis against normal human serum or human transferrin. Specific against human transferrin in electroblotting when tested against normal human serum.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Predicted molecular weight: 77 kDa.
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family. Contains 2 transferrin-like domains.