Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)

Overview

  • Product nameAnti-Transferrin antibody [EPR2932(2)] - Serum Loading Control
    See all Transferrin primary antibodies
  • Description
    Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Transferrin aa 100-200.

  • Positive control
    • Liver tissue. Fetal liver or prostate cancer lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
    • Sequence similaritiesBelongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control images

    • All lanes : Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/1000 dilution

      Lane 1 : fetal liver lysate
      Lane 2 : prostate cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 77 kDa
    • Immunohistochemical analysis of Transferrin expression in formalin-fixed, paraffin-embedded Human liver tissue, using ab109503 at 1/250 dilution.

    References for Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)

    ab109503 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"