Overview

  • Product nameAnti-Transferrin antibody (HRP)
    See all Transferrin primary antibodies
  • Description
    Sheep polyclonal to Transferrin (HRP)
  • ConjugationHRP
  • SpecificityThe unconjugated immunoglobulin gives a single arc when tested by immunoelectrophoresis and 2D-IEPagainst human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti-humantransferrin of known specificity.
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The immunogen used was Human Holo-Transferrin purified from human serum and shown to be homogeneousby SDS-PAGE.

Properties

Applications

Our Abpromise guarantee covers the use of ab9036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesFor enzyme immunoassays, to give an A450nm = 1.0 using wells coated with transferrin at 2µg/mLand TMB as a substrate: 1/12800 – 1/25600
    The titres reported should be used as guidelines for determining the working dilution in the userssystem.

    The following points should be observed when using this product:

    1) Dilutions of the conjugate must be made in a suitable buffer (e.g. 0.15M Phosphate buffered saline,pH7.2)
    2) It is recommended that dilutions of the conjugate are made in polypropylene or polyethylene containers.Other materials (e.g. polystyrene, some grades of glass) may be detrimental to the conjugate.
    3) Working dilutions of the conjugate should be prepared immediately prior to use.
    4) Peroxidase conjugates are adversely affected by the presence of sodium azide, the use of which shouldtherefore be avoided.
    5) The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent nonspecificbinding in EIA. It may also be advantageous in other assay systems.
  • Target

    • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
    • Sequence similaritiesBelongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    References for Anti-Transferrin antibody (HRP) (ab9036)

    ab9036 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"