Overview

  • Product nameAnti-Transferrin antibody [HTF-14]
    See all Transferrin primary antibodies
  • Description
    Mouse monoclonal [HTF-14] to Transferrin
  • SpecificityThis antibody recognizes an epitope located in the N-terminal domain of human transferrin.
  • Tested applicationsSuitable for: IHC-P, ELISA, RIA, WB, Sandwich ELISA, ICC, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Rabbit, Human, Pig
    Does not react with: Sheep, Horse, Cow, Dog, Carp
  • Immunogen

    Full length native protein (purified) (Pig).

Properties

Applications

Our Abpromise guarantee covers the use of ab769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Works best under non reducing conditions.

Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Transferrin (ab9538). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions.

Target

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apotransferrin antibody
    • Beta 1 metal binding globulin antibody
    • Beta-1 metal-binding globulin antibody
    • DKFZp781D0156 antibody
    • PRO1400 antibody
    • PRO1557 antibody
    • PRO2086 antibody
    • Serotransferrin antibody
    • Serotransferrin precursor antibody
    • Siderophilin antibody
    • TF antibody
    • TFQTL1 antibody
    • Transferin antibody
    • Transferrin antibody
    • TRFE_HUMAN antibody
    see all

Anti-Transferrin antibody [HTF-14] images

  • All lanes : Anti-Transferrin antibody [HTF-14] (ab769)

    Lane 1 : Transferrin – 8 µl (5 µg) (reducing conditions)
    Lane 2 : Transferrin – 5 µl (3 µg) (reducing conditions)
    Lane 3 : Transferrin – 3 µl (1 µg) (reducing conditions)
    Lane 4 : Transferrin – 3 µl (1 µg) (non reducing conditions)
    Lane 5 : Transferrin – 5 µl (3 µg) (non reducing conditions)
    Lane 6 : Transferrin - 8 µl (8 µg) (non reducing conditions)

    The sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.

    Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer

    SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl

    Marker – 3 µl

    WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes

    Detection:

    Blocking solution: 1xPBS/5% BSA, 4°C, overnight

    Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)

    Washing solution: 1x PBS/0.1% Tween

    Secondary Ab: IRDye®800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.

    Detection: Li-COR odyssey

    No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.

  • Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.

Protocols

References for Anti-Transferrin antibody [HTF-14] (ab769)

This product has been referenced in:
  • Salvati A  et al. Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface. Nat Nanotechnol 8:137-43 (2013). Read more (PubMed: 23334168) »
  • Pitek AS  et al. Transferrin coated nanoparticles: study of the bionano interface in human plasma. PLoS One 7:e40685 (2012). Dot blot . Read more (PubMed: 22829881) »

See all 4 Publications for this product

Product Wall

Lateral flow is not a tested application, however it is very much like sandwich ELISA so any ab pair used in sELISA should work in LF. We do offer a matched antibody for use with ab769 this is ab9538. Gold conjugation is generally used in LF. We d...

Read More

Thank you for contacting Abcam.

Since all three antibodies have IgG (or a IgG subclass) isotypes, the molecular weight of each is approximately 160kDa (23-25kDa for both light chains, and 53kDa for both heavy chains).

Application Western blot
Sample Human Tissue lysate - other (artery biopsy)
Loading amount 20 µg
Specification artery biopsy
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Feb 06 2007

Thank you for the requested information. I have re-examined our record and I was not able to find this information in our past correspondences with you. In regards to the purchase order number "192677", this was an order for ab7355 (Acinus antibody...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"