Anti-Transferrin Receptor 2 antibody (ab80194)

Overview

  • Product nameAnti-Transferrin Receptor 2 antibody
    See all Transferrin Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Transferrin Receptor 2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Transferrin Receptor 2.

    (Peptide available as ab87613.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysate: Human Liver

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).

Abcam recommends using 5% BSA as the blocking agent.

ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionMediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
  • Tissue specificityPredominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly.
  • Involvement in diseaseDefects in TFR2 are a cause of hemochromatosis type 3 (HFE3) [MIM:604250]. HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects.
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
  • Cellular localizationCell membrane and Cytoplasm. Lacks the transmembrane domain. Probably intracellular.
  • Information by UniProt
  • Database links
  • Alternative names
    • HFE 3 antibody
    • HFE3 antibody
    • MGC126368 antibody
    • TFR 2 antibody
    • TfR2 antibody
    • TFR2_HUMAN antibody
    • TFRC 2 antibody
    • TFRC2 antibody
    • Transferrin receptor 2 antibody
    • Transferrin receptor protein 2 antibody
    see all

Anti-Transferrin Receptor 2 antibody images

  • Anti-Transferrin Receptor 2 antibody (ab80194) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 98 kDa (possible glycosylated form).

    Exposure time : 5 minutes
    Transferrin receptor 2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
  • All lanes : Anti-Transferrin Receptor 2 antibody (ab80194) at 1/1000 dilution

    Lane 1 : Rat liver whole tissue lysate
    Lane 2 : Rat liver whole tissue lysate
    Lane 3 : Rat liver whole tissue lysate
    Lane 4 : Rat liver whole tissue lysate

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 88 kDa


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab80194 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80194, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Transferrin staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80194, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Transferrin Receptor 2 antibody (ab80194)

This product has been referenced in:
  • Naz N  et al. Ferroportin-1 is a 'nuclear'-negative acute-phase protein in rat liver: a comparison with other iron-transport proteins. Lab Invest : (2012). WB ; Rat . Read more (PubMed: 22469696) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 18 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 15 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (HepG2)
Specification HepG2
Permeabilization Yes - 0.3% Triton X
Fixative Methanol
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Submitted Nov 10 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12)
Sample Rat Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 18 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Rat Tissue sections (liver)
Specification liver
Permeabilization Yes - 0.3% Tween 20
Fixative Methanol
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Submitted Jul 09 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 04 2013

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 04 2013

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Tissue lysate - whole (Spleen)
Specification Spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jul 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Spleen)
Specification Spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jun 24 2013

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