The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
Tissue specificityPredominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly.
Involvement in diseaseDefects in TFR2 are a cause of hemochromatosis type 3 (HFE3) [MIM:604250]. HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects.
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
Cellular localizationCell membrane and Cytoplasm. Lacks the transmembrane domain. Probably intracellular.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymus tissue labelling Transferrin Receptor 2 with ab83810 at 5.0µg/ml.
References for Anti-Transferrin Receptor 2 antibody (ab83810)
This product has been referenced in:
Foka P et al. A complex signaling network involving protein kinase CK2 is required for hepatitis C virus core protein-mediated modulation of the iron-regulatory hepcidin gene expression. Cell Mol Life SciN/A:N/A (2014).
Read more (PubMed: 24718935) »
Zhou GL et al. Deficits in receptor-mediated endocytosis and recycling in cells from mice with Gpr107 locus disruption. J Cell Sci127:3916-27 (2014).
Read more (PubMed: 24849652) »