Anti-Transferrin Receptor antibody [13E4] (HRP) (ab10250)


  • Product nameAnti-Transferrin Receptor antibody [13E4] (HRP)
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [13E4] to Transferrin Receptor (HRP)
  • ConjugationHRP
  • SpecificityThis antibody reacts with human soluble transferrin receptor.
  • Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notesConcentration varies from lot to lot and can be provided on request.



Our Abpromise guarantee covers the use of ab10250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [10D2] to Transferrin Receptor (ab10247) and Mouse monoclonal [23D10 ] to Transferrin Receptor (ab10251).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in diseaseImmunodeficiency 46
    • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    References for Anti-Transferrin Receptor antibody [13E4] (HRP) (ab10250)

    ab10250 has not yet been referenced specifically in any publications.

    Product Wall

    Thanks for your enquiry.

    We do not have a specific ELISAprotocol to be used with ab10250. However we do have a number of good, general ELISA protocols that I'm sure you will find helpful. Here is a link to our protocols. Please note the ELISA...

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    Thank you for your response.

    The products that you have indicated will work with each other effectively in ELISA. These have been tested and are covered by our Abpromise for a period of 6 months if used according to the specifications on the d...

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    Thank you for contacting Abcam.

    I believe that you have inadvertently written ab10250 twice in this email. This product is an excellent choice for detection of TfR and has been conjugated to an HRP tag. Of the remaining antibodies which have b...

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