Anti-Transferrin Receptor antibody (ab84036)

Overview

  • Product nameAnti-Transferrin Receptor antibody
    See all Transferrin Receptor primary antibodies
  • Description
    Rabbit polyclonal to Transferrin Receptor
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Transferrin Receptor.

    (Peptide available as ab101219.)

  • Positive control
    • This antibody gave a positive signal in mouse spleen tissue lysate as well as the following human whole cell lysates: HeLa; Jurkat; U20S.

Properties

Applications

Our Abpromise guarantee covers the use of ab84036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-FrFl 1/200.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody images

  • All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ab84036 immunohistochemical staining in the rat brain. Sampled were fixed with formaldehyade and heat-mediated antigen retrieval was performed with citric acid. Tissue sections were blocked in 1% BSA for 10 minutes at 21°C before incubation with ab84036 (1/6000) for 16 hours at 21°C. A biotin conjugated goat anti-rabbit IgG secondary was used at 1/250.

    See Abreview

  • ICC/IF image of ab84036 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84036 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Spleen (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 50,100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
    Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ab84036 staining mouse liver sections by IHC-FrFl. The animal was perfused with 4% paraformaldehyde, further post fixed with 4% paraformaldehyde overnight and prepared for free floating sectioning. Staining with ab84036 at a 1/200 dilution in 0.1% TritonX with 0.1x PBS and 10% donkey was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody was used as the secondary antibody.

    See Abreview

  • ab84036 staining Transferrin Receptor in canine kidney cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.5% saponin then incubated with ab84036 at a 1/100 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.

    See Abreview

References for Anti-Transferrin Receptor antibody (ab84036)

This product has been referenced in:
  • Wang Y  et al. Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis. Cancer Sci 107:250-7 (2016). Rat . Read more (PubMed: 26679080) »
  • Song J  et al. Native low density lipoprotein promotes lipid raft formation in macrophages. Mol Med Rep 13:2087-93 (2016). WB ; Mouse . Read more (PubMed: 26781977) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Hamster Cell lysate - whole cell (BHKT7)
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Loading amount 250000 cells
Specification BHKT7
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted May 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization Yes - see blocking buffer
Specification undifferentiated Human ES cells on MEFs
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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Submitted Apr 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - other (Baby hamster kidney fibroblast cells (BHK-21))
Gel Running Conditions Reduced Denaturing (12 SDS-PAGE)
Loading amount 10 µg
Specification Baby hamster kidney fibroblast cells (BHK-21)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 29 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (undifferentiated Human ES cells on MEFs)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification undifferentiated Human ES cells on MEFs
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jan 15 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Nov 13 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK cells)
Permeabilization Yes - 0.1% DOC
Specification MDCK cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

馨 宮原

Verified customer

Submitted Oct 23 2015

Application Western blot
Sample Human Cell lysate - whole cell (MCF7 and MET5A cancer cell lines)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 100000 cells
Specification MCF7 and MET5A cancer cell lines
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Aug 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Placenta)
Specification Placenta
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 20 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 02 2014

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