Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)

Overview

  • Product nameAnti-Transferrin Receptor antibody [B349 (DF1513)]
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
  • SpecificityThis antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages.
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cell line KG1.

  • Positive control
    • Tonsil.
  • General notes


    This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.

Properties

Applications

Our Abpromise guarantee covers the use of ab8598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [B349 (DF1513)] images

  • All lanes : Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598) at 1/100 dilution

    Lane 1 : Human U2OS cell line, whole cell lysate
    Lane 2 : Human 293T cell line, whole cell lysate
    Lane 3 : African Green Monkey Cos7 cell line, whole cell lysate
    Lane 4 : Hamster CHO cell line, whole cell lysate
    Lane 5 : Rat PC12 cell line, whole cell lysate
    Lane 6 : Mouse 3T3 cell line, whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 7 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)

This product has been referenced in:
  • Ortiz-Zapater E  et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). Read more (PubMed: 17059402) »
  • Ayala P  et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). Read more (PubMed: 16309460) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (lung epithelial cells)
Specification lung epithelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% saponin
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry.

We have not tested these products (ab1086, ab84036, ab9179) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivity whatso...

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Thank you for your inquiry.


We have confirmedTFR expression in Hep G2, HEL 92.1.7 and TF-1 whole cell lysates using thefollowing conditions:

Load 50 ug of total protein
Blocking with 5% milk TBST for one hour at room tem...

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Thanks for following up with me. I would be happy to send you a vial of ab8598. I will just need to locate your initial order for ab1086 to process your request. Do you have the purchase order number or Abcam order reference number associated w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount 30 µg
Specification U2OS and 293T cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 02 2007

Further to contact with the source of this antibody I have determined that this antibody does not perform well by western blotting. Given that this antibody was detailed as working by western blotting on our datasheets I would like to firstly apolagise...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. Whilst we recommend that our customers use a tonsil preparation as a p...

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The epitope has not been mapped. The cellular loction is cell membrane.

Thank you for your enquiry. Here are some references: Testa, U., wt al 1993. Crit. Rev. Oncog. 4.241-276, Barclay, A.N., et al. 1997. Leucocyte Antigen FactsBook. Academic Press. London Oudemans, et al. 1986 Cancer 58. 1252. Hentze, M.W. and K...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"