Anti-Transferrin Receptor antibody [DF 1513 (Workshop IV)] (ab116015)

Overview

  • Product nameAnti-Transferrin Receptor antibody [DF 1513 (Workshop IV)]
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [DF 1513 (Workshop IV)] to Transferrin Receptor
  • Tested applicationsSuitable for: WB, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Transferrin Receptor.

  • Positive control
    • Human tonsil and placenta tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab116015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Detects a band of approximately 90-95 kDa (predicted molecular weight: 85 kDa).
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration. (methanol fixed).

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

References for Anti-Transferrin Receptor antibody [DF 1513 (Workshop IV)] (ab116015)

ab116015 has not yet been referenced specifically in any publications.

Product Wall

We do not have a CD71 antibody that has been tested in ELISA or any other kind of capture assay, but the monoclonal antibody ab116015 is specific for an extracellular epitope and can at least identify CD71 on cells. Capturing reticulocytes with the mag...

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Thank you for your email.

Unfortunately, no information about the use of a permeabilization step was found by the lab. Thus, it is not clear if the binding site is extra- or intracellular.

I am sorry to not have more helpfulinform...

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Thank you for contacting us.

I have heard back from the lab that the epitope of this antibody is not known. I have asked them to check if a permeabilization step is needed as this would tell us if the antibody binding site would be extracell...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"