Anti-Transferrin Receptor antibody [DF1513] (Biotin) (ab7350)


  • Product nameAnti-Transferrin Receptor antibody [DF1513] (Biotin)
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [DF1513] to Transferrin Receptor (Biotin)
  • ConjugationBiotin
  • SpecificityDF1513 recognises the human CD71 cell surface antigen, a 190 kD homodimeric glycoprotein.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    KG1 acute myeloid leukemia cell line



Our Abpromise guarantee covers the use of ab7350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow cytometry: neat. Use 10 µl to label 106 cells.
    Does not work in immunohistology on frozen and paraffin-embedded sections.
  • Target

    • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in diseaseImmunodeficiency 46
    • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    References for Anti-Transferrin Receptor antibody [DF1513] (Biotin) (ab7350)

    This product has been referenced in:
    • Ayala P  et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). Read more (PubMed: 16309460) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. Ab8598 and Ab7350 are the same clone, DF1513. We get these two antibodies from two different sources. Ab8598 has been tested for application in IHC-frozen sections. Unfortunately, we are not aware of any publications that fe...

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    Thank you for your enquiry. Any human cell line eg. KG1, U266, MOLT4 (i.e. anything that is proliferating well) would represent a suitable positive control for this antibody in flow cytometry.

    As we would not have tested the anti-human CD71 antibody in cell monolayers/smears we would be unable to guarantee its suitability for this procedure. However, having said that, fixing cells on slides and staining them is probably the most simple imm...

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